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superscript cdna synthesis kit  (TaKaRa)


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    TaKaRa superscript cdna synthesis kit
    Superscript Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+kit/SMARTer+PCR+cDNA+Synthesis+Kit/pm40921884-94-9-13
    Average 96 stars, based on 6067 article reviews
    superscript cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis.
    Article Snippet: For miRNA-seq, we collected MSC-Exos as previously described, and samples were sequenced on the Illumina sequencing platform by RiboBio Biotechnology Co., Ltd. (Guangzhou, China). .. For RT-PCR, retinal tissues were harvested immediately using TRIzol reagent (Takara), and total RNAs were extracted using phenol/ chloroform, from which 1 mg of RNA was reverse transcribed using the Superscript cDNA kit (Takara). .. The resulting cDNA was used as a template for subsequent PCR amplification with specific primers (Gene Company Limited).

    Article Title: Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis
    Article Snippet: For miRNA-seq, we collected MSC-Exos as previously described, and samples were sequenced on the Illumina sequencing platform by RiboBio Biotechnology Co., Ltd. (Guangzhou, China). .. For RT-PCR, retinal tissues were harvested immediately using TRIzol reagent (Takara), and total RNAs were extracted using phenol/chloroform, from which 1 mg of RNA was reverse transcribed using the Superscript cDNA kit (Takara). .. The resulting cDNA was used as a template for subsequent PCR amplification with specific primers (Gene Company Limited).

    cDNA Synthesis:

    Article Title: Brilliant blue G attenuates lipopolysaccharide-mediated microglial activation and inflammation
    Article Snippet: BV2 cells were treated and total RNA was extracted using an RNeasy mini kit [TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, Liaoning Province, China]. .. RNA samples were used for first-strand cDNA synthesis using a SuperScript cDNA kit (TaKaRa). .. Real-time PCR was performed using a Roche Lightcycle 480 (Roche, Welwyn Garden, Hertfordshire, UK) and a SYBR green real-time kit (TaKaRa).



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    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control